| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136537 |
| Chromosome: | chromosome_12 |
| Location: | 6142451 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536050 | ALA1 | Phospholipid-transporting ATPase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCCATGAAGCCCGTTGGGTGCGGCGTCC |
| Internal bar code: | GCTAGTTGCTTTTGTTTAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.8197 |
| LEAP-Seq n confirming: | 1661 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCACAATTGGGCCTATC |
| Suggested primer 2: | AACCTTGCCATTTTGCAATC |