Insertion junction: LMJ.RY0402.136569_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.136569
Chromosome:chromosome 8
Location:857832
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g361150 GT90F11,GT90-11 GT90 family protein 11; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTTGATGAGGCGTGACAGGTTCGGAAGG
Internal bar code:CATCAAGAAATGGGTTGTGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:74
LEAP-Seq percent confirming:98.0287
LEAP-Seq n confirming:2188
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATTAGTGTCCACACGCT
Suggested primer 2:CATGGAGTTCTGTGTGGGTG