| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136607 |
| Chromosome: | chromosome 10 |
| Location: | 6259386 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464850 | MSRA5 | (1 of 2) PTHR10173//PTHR10173:SF34 - METHIONINE SULFOXIDE REDUCTASE // SUBFAMILY NOT NAMED; Peptide methionine-S-sulfoxide reductase, msrA-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAACCCGGAGGCCCGGGCCGGGGTGAC |
| Internal bar code: | TCATGCACCGTGAAGACGCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 990 |
| LEAP-Seq percent confirming: | 99.6998 |
| LEAP-Seq n confirming: | 4981 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGAAGGAGGTACTGAGG |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |