| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136706 |
| Chromosome: | chromosome 5 |
| Location: | 3316900 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240400 | SOM2 | (1 of 2) PTHR11746//PTHR11746:SF104 - O-METHYLTRANSFERASE // O-METHYLTRANSFERASE 10; S-adenosyl-L-methionine-dependent O-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTACGACTACGCCTGCATACGAAGGGCTA |
| Internal bar code: | ACGTCCTGGGACCCGGTCGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 87.2979 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 110 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAAGACCGTACGCACTGCAC |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |