| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136706 |
| Chromosome: | chromosome 6 |
| Location: | 2776483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271050 | URN1 | (1 of 1) 3.2.2.3 - Uridine nucleosidase / Uridine ribohydrolase; Inosine/uridine-preferring nucleoside hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCGTGAAACTACCACACTCACTTCCAC |
| Internal bar code: | AATGCAGGTGCGCTTACAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 95.2663 |
| LEAP-Seq n confirming: | 644 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTACCTGCACGACCCAAC |
| Suggested primer 2: | GGGCGTTGGTGTAACAATTC |