| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136719 |
| Chromosome: | chromosome 14 |
| Location: | 680699 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612426 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTGACTTCGGGCTCCCGCGTCGGACGC |
| Internal bar code: | TATTTTCACGGCTGACGCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1115 |
| LEAP-Seq percent confirming: | 97.7865 |
| LEAP-Seq n confirming: | 1988 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACCACAACAAGAGAGCG |
| Suggested primer 2: | GGAACAACTTTTGGCACGAT |