| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136733 |
| Chromosome: | chromosome 2 |
| Location: | 624080 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077925 | (1 of 1) PTHR15628//PTHR15628:SF1 - FAMILY NOT NAMED // RWD DOMAIN-CONTAINING PROTEIN 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGGTACGTGTGGGGTCCAGCAGTGCCCT |
| Internal bar code: | TGCGACTTTGACACCGGACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 258 |
| LEAP-Seq percent confirming: | 77.6243 |
| LEAP-Seq n confirming: | 281 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGTGTGTGTGCGTGT |
| Suggested primer 2: | CCTTGGGTCACGATGAGTTT |