| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136733 |
| Chromosome: | chromosome 2 |
| Location: | 624110 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077925 | (1 of 1) PTHR15628//PTHR15628:SF1 - FAMILY NOT NAMED // RWD DOMAIN-CONTAINING PROTEIN 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGCACAGCGACAGAAGAGCTCTGGAGG |
| Internal bar code: | AAGGTTTTCCTGACACGTGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 99.0826 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGGGTCACGATGAGTTT |
| Suggested primer 2: | GTGTGTGTGTGTGTGCGTGT |