| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136741 |
| Chromosome: | chromosome 6 |
| Location: | 7313149 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298800 | ARS73A,ARS73a,LTH5 | Arylsulfatase 73a | intron|outside_mRNA |
| Cre06.g298802 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCCCAGGCGGGTGCTCGGAAGAACCGT |
| Internal bar code: | CCCGAATTCGATCAGCATCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1200 |
| LEAP-Seq percent confirming: | 99.5206 |
| LEAP-Seq n confirming: | 4775 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAGCAGGTGAGCATACG |
| Suggested primer 2: | TACAAGTGCGATGCCAGAAG |