| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136746 |
| Chromosome: | chromosome 7 |
| Location: | 2413931 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328800 | NSG13 | (1 of 8) PF00514 - Armadillo/beta-catenin-like repeat (Arm); Protein expressed during nitrogen-starved gametogenesis | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCCCCCCCCCCCCTTCTATTAAGACCC |
| Internal bar code: | GATTCGTGACTATGCATAAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 654 |
| LEAP-Seq percent confirming: | 99.4865 |
| LEAP-Seq n confirming: | 8138 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGACACAGCAGGATGAGA |
| Suggested primer 2: | CGGTCATACATCACGAGTGC |