| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136757 |
| Chromosome: | chromosome 1 |
| Location: | 169462 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGCCGCTGCCGCAGCCGCTGACGCAG |
| Internal bar code: | GGTTCAATACTCGCCGCCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 117 |
| LEAP-Seq percent confirming: | 60.0897 |
| LEAP-Seq n confirming: | 402 |
| LEAP-Seq n nonconfirming: | 267 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCACTGCTGCTTCATGTC |
| Suggested primer 2: | ATGCCAGACACCGAGGAG |