| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136766 |
| Chromosome: | chromosome 12 |
| Location: | 7774049 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553650 | (1 of 3) PTHR13887:SF10 - FRNE PROTEIN-LIKE | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCCCGCACCGCCGCGAACACACCCCTGC |
| Internal bar code: | GGAGGAGTGTTCTTCTTTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 99.1829 |
| LEAP-Seq n confirming: | 2792 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGTGTGGACACTAATCCC |
| Suggested primer 2: | TGGTGCTACATCTCAAAGCG |