| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136775 |
| Chromosome: | chromosome 17 |
| Location: | 642598 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700450 | GFY1 | (1 of 1) K07034 - uncharacterized protein (K07034); Putative acetate transporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGGACGCTGCGACGCGGGAATGGTGCC |
| Internal bar code: | TAAGCGTCGGCAAGTCATTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 233 |
| LEAP-Seq percent confirming: | 86.3748 |
| LEAP-Seq n confirming: | 13021 |
| LEAP-Seq n nonconfirming: | 2054 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAACGTCCACTAGGACGGG |
| Suggested primer 2: | GTACGTGAGGGAATCGTCGT |