| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136776 |
| Chromosome: | chromosome 12 |
| Location: | 8953795 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g544112 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTCCCCGTGAAAAATCCTTCAGTCGGTT |
| Internal bar code: | ATAGAATGCGCCGTTTTAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 90 |
| LEAP-Seq percent confirming: | 61.9718 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACACGTGAGGTGTACCA |
| Suggested primer 2: | GCTTTAGCGGAAGCCCTAGT |