| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136780 |
| Chromosome: | chromosome 3 |
| Location: | 2844086 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162500 | (1 of 4) IPR011016//IPR013083 - Zinc finger, RING-CH-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAAGGAGAAGGAAGAGGATGCTGGTTGG |
| Internal bar code: | ACGTCCACATCGCGTGGTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 99.4094 |
| LEAP-Seq n confirming: | 12625 |
| LEAP-Seq n nonconfirming: | 75 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATGTGTGCATTAAGGG |
| Suggested primer 2: | ATTAAAGCAGTCGGGCTGTG |