| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136784 |
| Chromosome: | chromosome 3 |
| Location: | 2031224 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156150 | HEL13 | (1 of 1) K14776 - ATP-dependent RNA helicase DDX10/DBP4 [EC:3.6.4.13] (DDX10, DBP4); DEAD/DEAH box helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGGCGGCGTCGAAGGCGGCGGCGGGGTC |
| Internal bar code: | GCAAAACTCCCGGAATATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 787 |
| LEAP-Seq percent confirming: | 98.7018 |
| LEAP-Seq n confirming: | 2813 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACCTCAACCTCACAGGG |
| Suggested primer 2: | CGTGGATCAAGAAAGGGTGT |