| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136788 |
| Chromosome: | chromosome 17 |
| Location: | 2974829 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720183 | (1 of 1) K08998 - hypothetical protein (K08998) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGTACCCCGTCCCTTCGCACCCACCAT |
| Internal bar code: | TGCATCTAGTAGAATGTATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1125 |
| LEAP-Seq percent confirming: | 99.0119 |
| LEAP-Seq n confirming: | 10722 |
| LEAP-Seq n nonconfirming: | 107 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCAGGGGGAGGTAAACT |
| Suggested primer 2: | GACAGCCGCTCTTTATTTGC |