| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136835 |
| Chromosome: | chromosome 16 |
| Location: | 7260023 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683035 | (1 of 2) IPR003593//IPR027417 - AAA+ ATPase domain // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTAAATTACCGCCGGTCCCGCTCAAGGTC |
| Internal bar code: | TAAGTTGGGCTCGACTACAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 99.3691 |
| LEAP-Seq n confirming: | 11970 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTACAGCTCGTGATGA |
| Suggested primer 2: | CGGTATTGCCTACCAGAGGA |