| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136841 |
| Chromosome: | chromosome 9 |
| Location: | 5478086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400256 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGAGGCAGAGCAAGCTGGAGCAAGTAA |
| Internal bar code: | ATGTGAACGCGAAAGGTCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 98.5989 |
| LEAP-Seq n confirming: | 10204 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGCTGCTGTTGCTACT |
| Suggested primer 2: | TAAGCGCTGACAGCTGAAGA |