Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.136842 |
Chromosome: | chromosome_13 |
Location: | 3741616 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589450 | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCTGAGCTATGTGAGCGACGTGGAGTGCTC |
Internal bar code: | AGATCGTGCGACACATCACATT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 325 |
LEAP-Seq percent confirming: | 98.8372 |
LEAP-Seq n confirming: | 595 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGTAGCTGAACTCCACCG |
Suggested primer 2: | AACATGCCTGGTTGTAAGCC |