| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136847 |
| Chromosome: | chromosome 6 |
| Location: | 3249599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276450 | (1 of 4) 3.1.2.6 - Hydroxyacylglutathione hydrolase / Glyoxalase II | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCGCGTGGGCATGGGTCGGCAGGAGG |
| Internal bar code: | CGGCCCAGGAACAACCGCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 709 |
| LEAP-Seq percent confirming: | 97.3647 |
| LEAP-Seq n confirming: | 16552 |
| LEAP-Seq n nonconfirming: | 448 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGAGGGATGTGTGATG |
| Suggested primer 2: | GTGTATTAGCAGTGGCCCGT |