| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136857 |
| Chromosome: | chromosome 6 |
| Location: | 5711065 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286750 | (1 of 2) PF15247 - Histone RNA hairpin-binding protein RNA-binding domain (SLBP_RNA_bind) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGATAGCGCTCGGACACCTCGGCACGGCT |
| Internal bar code: | ACCGGTCGCCGTGGTGCCTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 99.9352 |
| LEAP-Seq n confirming: | 1542 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTACGCGATCTGTTCCAA |
| Suggested primer 2: | CTGGTATCCGACCGTGTTCT |