| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136860 |
| Chromosome: | chromosome 10 |
| Location: | 3692408 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446000 | (1 of 1) PF01843//PF10358 - DIL domain (DIL) // N-terminal C2 in EEIG1 and EHBP1 proteins (NT-C2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGGATGTAACCCCCCCCCCCCCCCCCC |
| Internal bar code: | CGCCTAGGATATCGCAGCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 98.2937 |
| LEAP-Seq n confirming: | 4666 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACCTGTCAGAGTATGCG |
| Suggested primer 2: | TACAGGCACGGTATGTCCAA |