Insertion junction: LMJ.RY0402.136876_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.136876
Chromosome:chromosome 13
Location:95672
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g562550 ARH1 (1 of 1) 1.18.1.6 - Adrenodoxin-NADP(+) reductase / Nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase; NADP adrenodoxin-like ferredoxin reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGGGCAGGCGAGGGGAGGGGGGGAAGGA
Internal bar code:CGCACGGAAGGGGTGAATCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:88
LEAP-Seq percent confirming:60.3125
LEAP-Seq n confirming:193
LEAP-Seq n nonconfirming:127
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCAATCCTCGATGAAATGCT
Suggested primer 2:CCATCATAAACCTTGGGGTG