| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136884 |
| Chromosome: | chromosome 16 |
| Location: | 3892061 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690300 | (1 of 1) PTHR17130//PTHR17130:SF23 - MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCTGGGCGCGATGAGCAGCCATTCCA |
| Internal bar code: | CAGTAACCCACACGTTCTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1298 |
| LEAP-Seq percent confirming: | 99.2638 |
| LEAP-Seq n confirming: | 10786 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTTAGGGCTCATGCAAGC |
| Suggested primer 2: | CGCCTCACTTTGTCCCTATC |