| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.136888 |
| Chromosome: | chromosome 12 |
| Location: | 5432918 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g530250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCATGCGGATGATCCGTGGGCATTTCGG |
| Internal bar code: | AACTGTTCGGCAATATCCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 99.7826 |
| LEAP-Seq n confirming: | 4131 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGATAGCGGTGAAGGGTA |
| Suggested primer 2: | ACCATGGTTTGACGGAAGAG |