| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136918 |
| Chromosome: | chromosome_3 |
| Location: | 5077041 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g181150 | DLL1,ROC106,FLA14,RSP22,LC8 | Dynein arm light chain 8, LC8 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACTAGCAAAATGGCGTCGGGTAGCTCCAA |
| Internal bar code: | AGGGTGGCCATGGGTCGAGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 98.7906 |
| LEAP-Seq n confirming: | 4656 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTTCCTCCCCTTCCTTC |
| Suggested primer 2: | AAATGTATTTCGACCCTGCG |