Insertion junction: LMJ.RY0402.136932_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.136932
Chromosome:chromosome 6
Location:5436099
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g284250 LHCBM8 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGACGACCACCTGGCCAACCCTGGTGTG
Internal bar code:GCACGTGACTAGATCACCCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:799
LEAP-Seq percent confirming:99.6506
LEAP-Seq n confirming:1711
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TACAATTCCGCTCTGTGCTG
Suggested primer 2:ACAATCACACGAACGGTCAA