| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.136960 |
| Chromosome: | chromosome 6 |
| Location: | 1706401 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261400 | DTH1 | Delayed in Triacylglycerol Hydrolysis 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACCAAGGAGGATGAGGAGCGGGAGCGTG |
| Internal bar code: | TCGACGGATTAGCGGACCAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 98.7926 |
| LEAP-Seq n confirming: | 11210 |
| LEAP-Seq n nonconfirming: | 137 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACATACGGCAAGAAAGGT |
| Suggested primer 2: | TGATTCCATCAAAGACAGCG |