| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.136964 |
| Chromosome: | chromosome 1 |
| Location: | 780865 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004050 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACAAGAAAGTCACAAAACTGTCCGAGC |
| Internal bar code: | TCTCCGGGGAGGGTCCCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.3054 |
| LEAP-Seq n confirming: | 18873 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGTACTCAGAAGCCGTCG |
| Suggested primer 2: | CAGGCATAGCTATAAGCGGC |