| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136966 |
| Chromosome: | chromosome 7 |
| Location: | 236251 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313800 | CGL138 | Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTTGATTTATACTGTAGCTCGAGCCCC |
| Internal bar code: | GGAGCAGGACAGTCTCACTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 99.4709 |
| LEAP-Seq n confirming: | 3008 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAGTCACCTTCTGCCTG |
| Suggested primer 2: | CAAGACGAGCCCTAGTTTGC |