| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.136973 |
| Chromosome: | chromosome 1 |
| Location: | 7348772 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATACAGCTCGCGATCGCGCACCTAGCT |
| Internal bar code: | ATCTCGGCTGTGATCCTGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 92.4109 |
| LEAP-Seq n confirming: | 7367 |
| LEAP-Seq n nonconfirming: | 605 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATGGGGTTCTTTACTGT |
| Suggested primer 2: | GCCCATGAGGACAAAGACAT |