Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.136974 |
Chromosome: | chromosome 7 |
Location: | 2384091 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g328550 | VPS18 | Subunit of the VPS-C complex; (1 of 1) PF05131 - Pep3/Vps18/deep orange family (Pep3_Vps18) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGATAAGGCGCGCTCCGCTTGACACGGC |
Internal bar code: | CGTTAAGGACTGAGCATCGAAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 808 |
LEAP-Seq percent confirming: | 97.8251 |
LEAP-Seq n confirming: | 2159 |
LEAP-Seq n nonconfirming: | 48 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTTCGAGAGGAACCTGCTG |
Suggested primer 2: | CATCCTCCTCCTTTTCCTCC |