Insertion junction: LMJ.RY0402.136974_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.136974
Chromosome:chromosome 7
Location:2384091
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328550 VPS18 Subunit of the VPS-C complex; (1 of 1) PF05131 - Pep3/Vps18/deep orange family (Pep3_Vps18) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCGATAAGGCGCGCTCCGCTTGACACGGC
Internal bar code:CGTTAAGGACTGAGCATCGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:808
LEAP-Seq percent confirming:97.8251
LEAP-Seq n confirming:2159
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTTTCGAGAGGAACCTGCTG
Suggested primer 2:CATCCTCCTCCTTTTCCTCC