| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137010 |
| Chromosome: | chromosome 2 |
| Location: | 5750501 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g110450 | (1 of 2) PF06201 - PITH domain (PITH); Proteasome-interacting thioredoxin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTATATGGTAGGCAGCTTGTCACTGCG |
| Internal bar code: | AGGGCGTGGTGGAAGATTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 99.7099 |
| LEAP-Seq n confirming: | 2062 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTAGAACAAACATGCGGT |
| Suggested primer 2: | ATGCTGCTTGATGTGCTTTG |