Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.137020 |
Chromosome: | chromosome_16 |
Location: | 7115950 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
None |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATGAAAAGCGCAGCCAGGCGTGGGGGTCA |
Internal bar code: | GGGGACAGAGGGCTACGAGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 924 |
LEAP-Seq percent confirming: | 99.6017 |
LEAP-Seq n confirming: | 5251 |
LEAP-Seq n nonconfirming: | 21 |
LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACACACACCCAACACACACC |
Suggested primer 2: | CTGTCATAGACTGCTGCCCA |