| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137036 |
| Chromosome: | chromosome 2 |
| Location: | 7196965 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146250 | NFS1,NIFS1,CSD1 | (1 of 1) PTHR11601:SF34 - CYSTEINE DESULFURASE, MITOCHONDRIAL; Cysteine desulfurase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTACAAGCGGCAGGGGCGCTGCTACACA |
| Internal bar code: | GAGACTGCCATCATCGCGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 607 |
| LEAP-Seq percent confirming: | 97.3739 |
| LEAP-Seq n confirming: | 7416 |
| LEAP-Seq n nonconfirming: | 200 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGGCGGAGAAGGAGAAG |
| Suggested primer 2: | TGGACATCAAGAGCATCCAG |