Insertion junction: LMJ.RY0402.137058_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.137058
Chromosome:chromosome 17
Location:4514828
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g732566 (1 of 31) IPR016187 - C-type lectin fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACTCATTTAAGTATGCCAGTGACGGG
Internal bar code:CAGTACTCCGACCCCCCGCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:219
LEAP-Seq percent confirming:3.57692
LEAP-Seq n confirming:186
LEAP-Seq n nonconfirming:5014
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCTTGCTACATGCTGTG
Suggested primer 2:ACTTTCAAGCCTGTCTCCGA