| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137066 |
| Chromosome: | chromosome 4 |
| Location: | 3284065 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g226811 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGCTTCAGCGGAGCTGCTGCCAGGACTG |
| Internal bar code: | AGGCTTGAGTTAGGAGTCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 99.5898 |
| LEAP-Seq n confirming: | 1214 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCGAGACTTCTGGGAGC |
| Suggested primer 2: | CACCTCACAGACAAGCGAAA |