Insertion junction: LMJ.RY0402.137068_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137068
Chromosome:chromosome_13
Location:1616884
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g573500 FAP95 Flagellar Associated Protein antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):ATCACAAGTGGCGAGCGCGACAGGGCGGAC
Internal bar code:GACGCCATACTATGGTAGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1265
LEAP-Seq percent confirming:99.8027
LEAP-Seq n confirming:6577
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CACCACTCCGACGGTAACTT
Suggested primer 2:AGACACAGAGGGAGGCTCAA