| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137068 |
| Chromosome: | chromosome 13 |
| Location: | 1616884 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573500 | BUG12,FAP95 | Flagellar Associated Protein 95 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACAAGTGGCGAGCGCGACAGGGCGGAC |
| Internal bar code: | GACGCCATACTATGGTAGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1265 |
| LEAP-Seq percent confirming: | 99.8027 |
| LEAP-Seq n confirming: | 6577 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACTCCGACGGTAACTT |
| Suggested primer 2: | AGACACAGAGGGAGGCTCAA |