| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137070 |
| Chromosome: | chromosome 7 |
| Location: | 1125403 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320250 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGAAGTCATTGCACACAGCCTGGCTAGT |
| Internal bar code: | CTCTGAGGACGGCCCAGTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1216 |
| LEAP-Seq percent confirming: | 99.6361 |
| LEAP-Seq n confirming: | 24919 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGACCAGGAACCCCTTA |
| Suggested primer 2: | CCTTAACCGAGAGTCAAGCG |