| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137073 |
| Chromosome: | chromosome 6 |
| Location: | 2388182 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g267850 | PLS1 | Phospholipid scramblase; (1 of 1) IPR005552//IPR025659 - Scramblase // Tubby C-terminal-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGCGCCGTCCTCCACAAACATGGTGG |
| Internal bar code: | GAAATCGGAAGCCTCGGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 237 |
| LEAP-Seq percent confirming: | 8.82353 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 1147 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGGCATGTCGCTAGCTC |
| Suggested primer 2: | CCAGTGTGGATACCATGCAG |