| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137077 |
| Chromosome: | chromosome 10 |
| Location: | 901800 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424250 | BUG13,FAP90 | Flagellar Associated Protein 90 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATTTTATAGCTGCCCGCTAACCGCGCA |
| Internal bar code: | GGTGAGCGGCTCATCTTTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 81.5789 |
| LEAP-Seq n confirming: | 310 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTAGAGGCACCTGTAGGA |
| Suggested primer 2: | CCTTCAACCAACACCCAACT |