| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137085 |
| Chromosome: | chromosome 5 |
| Location: | 915858 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247250 | (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACGTGATGACGTAAACGCAATCGATCA |
| Internal bar code: | CCGCTTTGCGGTTGGTCCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1022 |
| LEAP-Seq percent confirming: | 97.3677 |
| LEAP-Seq n confirming: | 7583 |
| LEAP-Seq n nonconfirming: | 205 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCAGAATTGTTGCAGAA |
| Suggested primer 2: | AGACAGCACCTCTCCCTCCT |