Insertion junction: LMJ.RY0402.137087_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.137087
Chromosome:chromosome 4
Location:3227847
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g226550 ARS11 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTACGGTTCACTTGGTGTGGCGTCGTAC
Internal bar code:CGAGCATCAGATCGTGCGTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:749
LEAP-Seq percent confirming:99.3882
LEAP-Seq n confirming:18195
LEAP-Seq n nonconfirming:112
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTTGTTTCCTTCCTTGCC
Suggested primer 2:CCATCATAAACACTGGCGTG