| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137089 |
| Chromosome: | chromosome 2 |
| Location: | 6362547 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115400 | (1 of 2) IPR002889//IPR008979 - Carbohydrate-binding WSC // Galactose-binding domain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCATGCGCGCATCCATGAATGTCTTCC |
| Internal bar code: | CATGGTCGGGCTGCAGCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.1639 |
| LEAP-Seq n confirming: | 5337 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTCTGCAAGCGTATGAT |
| Suggested primer 2: | GGGTTGCACGCTGGTATAGT |