Insertion junction: LMJ.RY0402.137113_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.137113
Chromosome:chromosome 3
Location:3190108
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g165300 (1 of 1) PF13350 - Tyrosine phosphatase family (Y_phosphatase3) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGCCTCCAGCACCCTCCACCTCTCCAC
Internal bar code:CGCTCGGATGATGGGCCCTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:427
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAGAGGCGTGTGTGTGTG
Suggested primer 2:CCACAACTCACAACACCTGC