| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.137119 |
| Chromosome: | chromosome 8 |
| Location: | 4238396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g380650 | GT90-13,GT90F13 | GT90 family protein 13; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCAGGAGGTCGCGCTGAATGCACGCCT |
| Internal bar code: | GGGCGCCTAATATCAGCTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 99.337 |
| LEAP-Seq n confirming: | 6892 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTATGTCGTTGCCAGGT |
| Suggested primer 2: | TGCACGTCTGATCTAGGTGG |