| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.137130 |
| Chromosome: | chromosome 6 |
| Location: | 2804272 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271200 | PNO1 | (1 of 1) 1.6.3.3 - NADH oxidase (H(2)O(2)-forming) / H(2)O(2)-forming NADH oxidase; Pyridine nucleotide-disulphide oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGGGGTGGGGCCGGGGCGCGGGGAGT |
| Internal bar code: | GGCACTTCTTAAACAACGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 99.8035 |
| LEAP-Seq n confirming: | 6602 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCACACAAACACAATACC |
| Suggested primer 2: | CCATTGCGTTCGGTTTAGTT |