| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.137150 |
| Chromosome: | chromosome 3 |
| Location: | 299918 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144607 | (1 of 79) IPR011992 - EF-hand domain pair | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACGGAACACAACCGCAACAGATATGGG |
| Internal bar code: | CCGCCAGTCTAAGCGAGCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 61.4865 |
| LEAP-Seq n confirming: | 455 |
| LEAP-Seq n nonconfirming: | 285 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCGAGGTGGGGAGGTA |
| Suggested primer 2: | TTTCGAGACGCATTCTCCTT |