Insertion junction: LMJ.RY0402.137152_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.137152
Chromosome:chromosome 16
Location:6566024
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g672350 (1 of 1) IPR001357//IPR001841 - BRCT domain // Zinc finger, RING-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACGCTGTGGAAATGCAGGTAGGCATTGT
Internal bar code:AGTCGTGAATCGCAATCACTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:60
LEAP-Seq percent confirming:83.7349
LEAP-Seq n confirming:139
LEAP-Seq n nonconfirming:27
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTCATTCGCTCCTGTAAC
Suggested primer 2:CGGGTGCTGACAAGGAGTAT